Catalogue K1209
From Gram positive and Gram negative bacteria
The Kit contains:
-50 HIGHWAY extractions /purifications (K1209-50)
It includes silica membrane mini columns, collection tubes, buffers, K proteinase and instructions manual. It does not include lysozyme.
IN VITRO RESEARCH USE
DNA Puriprep B-kit enables a quick and simple extraction and purification of genomic DNA from bacteria.
A pure DNA is obtained free of proteins, eluted from a silica mini column with RNA, which can be digested with RNase at will. The final product can be applied immediately to PCR or stored at -20ºC.
After the bacterial lysis with proteinase K and lysozyme (in the case of Gram positive), the process demands no more than 30-35 minutos and does not require organic solvents or phenol.
DNA Puriprep B-kit is transported at room temperature. Lyophilized proteinase K is delivered in a box; once received, keep at 4°C. After resuspension, keep at -20°C.