DNAse I (RNAse Free)
Catalogue K1212
PRESENTATION
The Kit contains:
- 1 vial with 1.000 U (1U/µl) of DNAse I, RNAse free (E1408)
- 1 vial with 1,2 ml of Reaction Buffer 10X (B0600)
- 1 vial with 1,2 ml of Stop Solution 10X (B0601)
IN VITRO RESEARCH USE
DESCRIPTION
DNAse I (RNase free) is a recombinant endonuclease which digests DNA nonspecifically and releases dinucleotides, trinucleotides and oligonucleotides with 5´-phosphorylated and 3´-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids. The enzyme comes from a recombinant clone of Pichia pastoris (PM 29 kDa).
Application: degradation of template DNA post in vitro transcription, RNA purification, DNAse I footprinting, nick translation, production of DNA fragments of random length.
Activity definition: 1 enzyme unit completely degrades 1 µg of plasmid DNA (pUC19) in 10 minutes at 37°C in a buffer with 50 mM of Tris-HCl (pH 7,8), 10 mM of MgSO4 and 1 mM of CaCl2.
Quality Control: DNA digestion after in vitro transcription, no ribonuclease activity.
Inhibitors: bivalent metal chelates, transition metals in mM concentration (e.g.: Zn2+, Co2+), SDS even in concentrations lower than 0,1%, reducing agents like DTT and ?-mercaptoethanol and ionic strength higher than 50-100 mM.
CONSERVATION
This product is transported with cooling gels and not with dry ice. This is due to the results of stability assays performed in the laboratory which allowed us to conclude that our product has a better performance under these conditions. Once received, keep at -20°C.
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